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DNA replication sites
The genome is a highly organized and compartmentalized structure. Standard microscopy techniques have enabled the visualization of replication sites, but with a diffraction-limited resolution of ~250nm. In this note, we describe how single molecule localization microscopy can be a powerful tool to study DNA replication sites.
Spectral demixing uses a single laser to excite spectrally close fluorophores. This technique eliminates several problems, including color cross-talk, chromatic aberration effects, and problems with drift between sequential color acquisitions.
Blinking pad kit
Single molecule imaging experiments are very sensitive to any movement, and therefore require cells to be immobile. In this context, abbelight developed a commercial kit for cell immobilization: the Blinking Pad kit
SMLM and microbiology
Because the size of bacterial cells is particularly small, single molecule microscopy is particularly well-adapted for revealing subcellular structures and dynamics that cannot be resolved with classical light microscopy...
Biological structures often form clusters. Determining where these clusters are in the cells, their size and density, and whether these parameters change in certain biological conditions are powerful tools that require specialized analysis methods...
Stochastic optical reconstruction microscopy (STORM) is based on the localization of individual fluorescent molecules. This separation in time and space can be achieved by using organic fluorophores and photoswitching buffers. In this context, abbelight developed a commercial STORM buffer, the Smart kit, for optimized and reproducible blinking.