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DNA replication sites
The genome is a highly organized and compartmentalized structure. Standard microscopy techniques have enabled the visualization of replication sites, but with a diffraction-limited resolution of ~250nm. In this note, we describe how single molecule localization microscopy can be a powerful tool to study DNA replication sites.
Stochastic optical reconstruction microscopy (STORM) is based on the localization of individual fluorescent molecules. This separation in time and space can be achieved by using organic fluorophores and photoswitching buffers. In this context, abbelight developed a commercial STORM buffer, the Smart kit, for optimized and reproducible blinking.