Single-Molecule Localization Microscopy
One-page technology notes on nanoscopy:
from sample preparation to imaging and data analysis, familiarize yourself with SMLM in an instant
In this page, you will find notes on different technologies related to nanoscopy.
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Technology notes
DNA replication sites
The genome is a highly organized and compartmentalized structure. Standard microscopy techniques have enabled the visualization of replication site, but with a diffraction-limited resolution of ~250nm. In this note, we describe how single molecule localization microscopy can be a powerful tool to study DNA replication sites.
Spectral demixing
Spectral demixing uses a single laser to excite spectrally close fluorophores. This technique eliminates several problems, including color cross-talk, chromatic aberration effects, and problems with drift between sequential color acquisitions.
Blinking pad kit
Single molecule imaging experiments are very sensitive to any movement, and therefore require cells to be immobile. In this context, abbelight developed a commercial kit for cell immobilization: the Blinking Pad kit
SMLM and microbiology
Because the size of bacterial cells is particularly small, single molecule microscopy is particularly well-adapted for revealing subcellular structures and dynamics that cannot be resolved with classical light microscopy...
Clustering
Biological structures often form clusters. Determining where these clusters are in the cells, their size and density, and whether these parameters change in certain biological conditions are powerful tools that require specialized analysis methods...
Single-particle tracking
SPT is a popular technique to monitor the dynamics of individual molecules, to distinguish populations with different dynamics, to evaluate diffusion coefficients...